Topflash实验原理
WebM50 Super 8x TOPFlash Sequences (3) Addgene Sequences: Full (1) Partial (2) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). ... WebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is …
Topflash实验原理
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WebTOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room temp for 5 min respectively. Both solutions are then mixed and incubated for another 15 min. Then, the mixture is... Web用western和免疫荧光验证beta－catenin和下游靶基因的变化只是基础，只是说明你转染了这个抑癌基因的确是改变了catenin通路，但是你想要证明该抑癌基因是通过catenin通路来 …
Web100μg. 1169.00元. FOPFlash(报告基因质粒)是TOPFlash(D2501)的阴性对照报告基因质粒。. FOPFlash质粒是以碧云天的pGL6-TA为模板，在其多克隆位点插入突变的TCF/LEF结合位 … Web1. sep 2015 · 1.应该是要用Top/FOP值来表示结果，Fop值当作一个背景值。 2.Fop是比较稳定的，特别是在组内。 不过你说“各组之间”，有可能不同处理会有影响，不过应该不影响结果分析。 一般Top会比Fop高或相似，除非你的处理是抑制Wnt通路的。 3.如果Fop值有降低，应该不是Wnt通路的改变引起的。 Top和Fop除了Wnt的反应元件不同，其余都一样， …
Webtopflash tcf lef luciferase reporter construct - by Bioz Stars , 2024-04 86 / 100 stars Images 1) Product Images from "Small molecule inhibitors of WNT/β-catenin signaling block IL-1β- and TNFα-induced cartilage degradation" Article Title: Small molecule inhibitors of WNT/β-catenin signaling block IL-1β- and TNFα-induced cartilage degradation WebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. …
WebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is activated, beta-catenin will translocate to the nucleus to associate with TCF/LEF transcription factors to activate transcription of Wnt target genes.
WebTOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room temp for 5 min respectively. Both solutions are then mixed and incubated for another 15 min. Then, the mixture is... blue bird white cityWeb9. dec 2024 · 双萤光素酶实验的原理 将目的基因转录调控原件构建入带有荧光素酶 (Firefly luciferase)的表达载体，构建成报告基因质粒，使这段序列调控luciferase的转录表达。 … blue bird winter migrationWeb22. sep 2015 · 一般而言，topflash/fopflash是比较常用的检测β-catenin的系统，原理和所有的RLU一样，都是利用转录因子可以和特异性的靶位点结合进而启动下游的虫荧光素酶基 … blue bird white breastWebPlasmid M50 Super 8x TOPFlash from Dr. Randall Moon's lab contains the insert TCF/LEF binding sites and is published in Curr Biol. 2003 Apr 15. 13(8):680-5. This plasmid is available through Addgene. blue bird wireless websiteWeb31. aug 1993 · SIST EN 60604:2003. 01-marec-2003. Topflash/Flipflash photographic flash lamp array (IEC 60604:1980) 'Topflash/Flipflash' photographic flash lamp array. Blitzlampensystem 'Topflash/Flipflash'. Dispositif 'Topflash/Flipflash' de lampes 'éclair' pour photographie. Ta slovenski standard je istoveten z: EN 60604:1993. free human anatomy gameWeb21. feb 2012 · 原理： 经典的Wnt信号通路需要beta-catenin入核，进而与转录因子TCF/LEF结合形成复合物，共同起始下游调控基因的转录。 据此，科学家通过将数个拷贝 … bluebird windows and doors lafayette coWebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. FOPFlash is similar, except that the Tcf-binding sites are mutated and non-functional. Thus, FOPFlash, which is a control reporter, yields expression from the bluebird wine emmylou harris